Tsui-Fen Chou, PhD
Assistant Professor of Pediatrics, David Geffen School of Medicine at UCLA
Division of Medical Genetics, Harbor-UCLA Medical Center
Developing new therapeutic targets for cancer
Research DescriptionDr. Chou's lab employs a combination of chemical biology, quantitative proteomics, high throughput, high-content screenings, and biochemical reconstitution to study the mechanism by which p97 orchestrates two major protein clearance pathways, proteasome and autophagy, and regulates the activity of proteins involved in apoptosis. In particular, Dr. Chou is using their novel p97 inhibitors in combination with genetic inhibition by RNAi or a dominant negative p97 mutant in proteomic experiments to identify substrates and investigate cell biological functions of p97. This work will generate important biomarkers for evaluating the harmacodynamics of the anti-cancer effects of p97 inhibitors in future animal studies and will facilitate the development of novel therapeutic agents.
- BS, 1999, School of Pharmacy, National Taiwan University, Taipei, Taiwan
- PhD, 2006, University of Minnesota, Twin Cities
Recent and/or Significant Publications
Gui, L , Zhang, X, Li, K., Frankowski, K., Shan Li, Wong, D., Moen, D.R, Porubsky, P.R., Lin, H. J., Schoene, F.J., Chou, T.-F. “Evaluating p97 Inhibitor Analogues for Potency against p97-p37 and p97-Npl4-Ufd1 Complexes.” ChemMedChem, 2016, 11, 953-957.
Zhang, X., Gui, L., Zhang, X., Bulfer, S. L., Sanghez, V., Wong, D., Lee, Y.J., Lehmann, L., Lee, J. S., Shih, P.-Y., Lin, H. J., Iacovino, M., Weihl, C. C., Arkin, M. R., Wang, T., Chou, T.-F. “Altered cofactor regulation with disease associated p97/VCP mutations.” Proc. Natl. Acad. Sci. USA, 2015, 112, E1705-1714.
Fang, C., Gui, L., Zhang, X., Moen, D.R., Li, K., Frankowski, K. J., Lin, H.J., Schoene, F.J., and Chou, T.-F. “Evaluating p97 inhibitor analogues for their domain-selectivity and potency against the p97-p47 complex” ChemMedChem, 2015, 10, 52-56. (PMID: 25377500)
Kato, M., Chou, T.-F, Yu, C.Z., Demodena, J., and Sternberg, P.W. “"LINKIN, a new transmembrane protein necessary for cell adhesion” eLife. 2014, e04449. (PMID:25437307)